Phytolith analysis is routinely utilized in archaeological contexts to answer a number of research questions ranging from agricultural strategies, to a community’s activity areas, to household plant use. While results can be informative and valuable, our understanding of plant use within occupation areas is heightened when they are paired with internal and external control samples from the surrounding areas. This study examines phytoliths from twenty one sediment samples extracted from four stratigraphic columns from the Jezreel Valley of modern-day Israel. Following standard techniques, we reconstruct the paleoenvironment of this large valley area to assess preservation conditions and summarize the past’s dominant plant communities. These results set a baseline understanding of phytolith densities and types to then examine with and in comparison, to three archaeological sites that have undergone previous phytolith analysis. Sites include Tel Shimron, Tel Megiddo, and Legio and will be completed at a later date. Together, this work provides a large scale understanding of the ancient Jezreel Valley and highlights the importance of collecting and analyzing external and internal control samples for meaningful microbotanical interpretations. This work is being completed by Atlatl Archaeology Ltd for the Jezreel Valley Regional Project, Tel Megiddo Expeditions, and Tel Shimron Excavations.